This is the classic anime plea 'yamete kudasai' (please stop) from Nichijou, usually delivered with exasperation or desperation. It's become a popular meme sound that people use when something ridiculous is happening and they want it to end, often in a comedic way.
Nіbutаnі Shіnkа – Yamеtе kudаsаі Sound Effect
Shared by Krabby